The waste produced in the course of healthcare activities carries a higher potential for infection and injury than any other type of waste. Inadequate and inappropriate knowledge of the handling of healthcare waste may have serious health consequences and a significant impact on the environment as well. This study aims to see whether PTP (Planned Teaching Programme) has significant impact on the level of knowledge and expressed practices of sanitary workers regarding bio-medical waste management, relationship between the level of knowledge and expressed practices and to seek the association between knowledge scores with selected demographic variables. The experimental approach one, group pretest post design and non-Probability purposive sampling technique was applied and data was collected by using the self report interview technique. The collected data were analyzed at it shows that there was a marked gain in the mean knowledge score (73.31%) and expressed practice (72.10%) after PTP. There was a positive relationship seen between the level of knowledge and expressed practice at 0.05 levels (t=0.273). There was no significant association found between selected demographic variables (age, gender, and work experience, the present area of work, monthly income and source of information) with the knowledge score of nurses regarding Bio Medical Waste Management.