Nurses are facing many challenges in this competitive era in health care settings and the one biggest challenge among the others is the application of theoretical concepts into practice. Moreover, the knowledge about nursing theory and its application into clinical practice is the hallmark of evidence-based practice, which is the focus of today's health care environment. The purpose of writing this paper is to show explicitly, the close relationship of nursing theory into clinical practice in order to prove evidence-based practice. Florence Nightingale is regarded as a pioneer in the nursing profession. Nightingale has given her notes on nursing in the form of theory of environment which is very useful for nurses to apply those concepts into clinical practice in order to improve the health care standards. All the major concepts of Nightingale's theory have been incorporated into the clinical scenario.