The current investigation considers the microstructural and mechanical properties of austenitic stainless steel SS316L through experimental investigation. The two metallic cylindrical specimens of SS316L were first fabricated using a conventional lathe machine. These parts were then machined to prepare tensile test specimens in accordance with IS 1608 Part-1:2022. After the specimens were prepared, their mechanical properties—yield strength, tensile strength, and elongation—were tested. All tests were carried out using a Universal Testing Machine under controlled conditions. Scanning Electron Microscopy (SEM) was also taken to assess the grain structure, porosity, and surface feature characteristics of SS316L. In the end, the SEM analysis confirmed that the two samples were different. It also revealed noticeable microstructural variations, which resulted from their earlier mechanical working and processing techniques. Sample 1 was a normal equiaxed structure, similarly as in annealed typical steel. Sample 2 highlighted both elongated grains and a particular deformation structure that indicates prior mechanical working. The mechanical performance of the samples also showed differences in both strength and ductility. This confirms that the initial preparation techniques can influence the final properties of SS316L. The scope of this study offers a direct comparison between the structure and properties of SS316L. It may also help improve understanding of how machining time and the type of mechanical treatment affect stainless steel components. Such insights are useful when designing performance characteristics for engineering applications.