Comparative Study of RDT and Elisa Kit for Diagnosis of Dengue Virus Infection

Shrawan Kr. Singh*, Rajeshwar Prasad Chourasia**
*-** Quest Pharmaceuticals Pvt. Ltd, Birgunj, Nepal.
Periodicity:July - September'2025

Abstract

Dengue fever (DF) is a viral disease generally transmitted by mosquitoes of the Aedes genus: Aedes aegypti. A total of 261 samples were tested by IgM Capture ELISA and Dengue IgM/IgG RDT Kit, out of which 18.8% of dengue-positive cases were detected by IgM Capture ELISA and 15.3% by the RDT Kit. The sensitivity of the RDT Kit was found to be 73.46% in comparison to ELISA. Among the suspected cases, 67.4% were male and 32.6% were female. The age group 15–50 years had the highest dengue-positive cases (81.6%). Students had the highest number of positive cases (32.7%) in the professional group. Patients with joint pain, retro-orbital pain, and skin rash as major symptoms were diagnosed as dengue-positive by RDT and ELISA test methods. The study focused only on dengue-positive or negative cases among suspected patients, but there is a need for molecular tests such as PCR for identification of serotypes circulating among suspected cases.

Keywords

DVI, IgM Capture, ELISA, RDT, Clinical Features, PCR.

How to Cite this Article?

Singh, S. K., and Chourasia, R. P. (2025). Comparative Study of RDT and Elisa Kit for Diagnosis of Dengue Virus Infection. i-manager’s Journal on Nursing, 15(2), 16-21.

References

[7]. Pandey, B. D., Rai, S. K., Morita, K., & Kurane, I. (2004). First case of Dengue virus infection in Nepal. Nepal Medical College Journal, 6(2), 157-159.
[9]. Sah, O. P., Subedi, S., Morita, K., & Pandey, B. D. ( 2009). Serodiagnosis of dengue by particle agglutination assay. Journal of Nepal Health Research Council, 7(1), 29-32.
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