The rapid evolution of digital electronics has transformed how embedded systems interface with personal computers and industrial equipment. Among various communication protocols, the Universal Serial Bus (USB) stands out for its versatility, speed, and plug-and-play functionality. However, in applications where electrical noise, ground potential variations, or high voltages exist, such as industrial automation or medical instrumentation, direct USB connections can compromise safety and signal integrity. This paper presents the design and evaluation of a galvanically isolated USB interface that provides reliable communication between embedded devices and host systems while maintaining electrical isolation. The proposed design integrates a data isolation stage using ISOUSB111 and an isolated DC–DC converter for power separation. Together, these ensure complete protection against transient voltages, electrostatic discharge (ESD), and ground loops. Complying with IEC 60601-1 medical safety standards, the design achieves robust isolation suitable for medical and industrial environments. Experimental validation demonstrates stable USB 2.0 full- speed data transfer (12 Mbps) and reinforced insulation capable of withstanding 5 kV AC potential difference.