The current laboratory study was to investigate the effect of steam sterilization treatments on anodized Titanium Nails of same length, operations and geometry using fixed sterilization process parameters (temperature 121°C, pressure 15 Psi and autoclaving time 30 minutes) for examine the colour fed issue after autoclave processing for 3 cycles. During sterilization the two Nail samples were used sterile cloth and other 2 samples used aluminium foil. All the samples were checked visually. Every cycle total four samples were used. Finally, all the samples were analysed visually.