This research paper explores the qualitative analysis of protein samples using the Biuret reagent through microscale methods involving ceramic tiles, grooved tiles, and Eppendorf tubes. The study highlights the advantages of minimal reagent usage, reduced waste, and enhanced safety. The Biuret test is employed to detect peptide bonds indicative of protein presence. Protein concentrations used for the identity assay ranged from 0.1% to 1.0%. Protein was detected by all three methods compared; however, the Eppendorf method detected proteins at a concentration of 0.3% and above. The ceramic and grooved tiles could detect proteins from 0.4% and above, demonstrating that the Eppendorf method was more sensitive. The microscale adaptation of this traditional test is evaluated for its efficacy, precision, and practicality in various applications, particularly in educational and resource-limited laboratory settings.