The demand for high-performance antennas tailored for 5G applications has stimulated research into novel designs and optimization techniques. This study focuses on the design, development, and optimization of microstrip patch antennas (MPAs) integrated with slots to enhance their performance in 5G communication systems. Various slot configurations, including rectangular and T-shaped slots, are incorporated into the ground plane of the patch antennas to investigate their impact on antenna characteristics such as gain, bandwidth, and radiation pattern. Simulation results demonstrate that the introduction of slots effectively increases the antenna's effective aperture, modifies current distribution, and enhances radiation efficiency. Furthermore, the strategic placement of slots improves antenna directivity and suppresses surface waves, leading to a notable improvement in antenna gain. Experimental validation corroborates the simulation findings, showcasing the practical feasibility and efficiency of the proposed slot-integrated antenna designs. Overall, this research contributes to the advancement of antenna technology tailored for the evolving requirements of 5G communication systems.