Interfaces, where different fluids meet, are crucial zones influencing processes like emulsification and foam creation. Understanding their properties is vital in chemical, biosciences and oil industry. Rheology, evaluating stress and strain, helps characterize these properties, distinguishing between material behavior like elasticity and viscosity. Stress causes deformation, crucial in defining a material's response. Elastic materials return to shape after stress, maintaining ratios. Interfacial studies benefit diverse fields like engineering, biology, and medicine, unveiling unique properties for material design. In biology, studying bio-interfaces elucidates complex cell behaviors and drug delivery. Recent research emphasizes the importance of studying interfaces. Peptide interfaces exhibit self-organization in cell elongation, while motor protein-based nano-biodevices and antimicrobial implant surfaces demonstrate new applications. Interfacial studies impact nanocomposites, dental materials, cancer stem cells, and nanospheres, showcasing their broad implications in diverse fields. Nanotechnology combined with biology creates new medical possibilities. Nanomedicine, using nanoparticles, shows promise in drug delivery and cancer treatment. Challenges exist in understanding bio-interfaces, crucial for medical progress. Advances in nanotech and interfacial studies could transform fields with ongoing research needed for big changes.