This study focuses on the preparation and characterization of the [Fe III(EDTA)H2O]- complex at low temperatures, with the objective of identifying heme-like water-soluble properties. The research explores the potential health benefits of this complex, extracted from natural sources, in enhancing immunity and leveraging its heme-like properties. The prepared water-soluble complex was subjected to a comprehensive characterization process utilizing various analytical techniques, including UV-Visible spectroscopy, Powder X-ray Diffraction (PXRD), and Fourier-Transform Infrared (FT-IR) spectroscopy. The characterization results revealed the compound's structural properties, optical behavior, and molecular interactions, providing valuable insights for its potential applications in various fields. The complex was also evaluated for its photocatalysis capabilities. The results of the study indicate that the prepared [Fe III(EDTA)H2O]- complex exhibits structural and spectral similarities to Hemin Iron(III) complex. This suggests that the [Fe III(EDTA)H2O]- complex could potentially have similar biological or catalytic properties to the Hemin Iron(III) complex, opening up new avenues for research and applications. This research lays the foundation for further investigations into the health benefits and potential applications of this heme-like water-soluble complex in immunology and photocatalysis.