This research presents a novel approach for effectively optimizing growth rate and yield in a greenhouse. With the market demanding higher-quality standards, greenhouse cultivation is becoming increasingly sophisticated and competitive. However, the implementation of protected cultivation systems is expensive. Therefore, it is crucial for African Indigenous Vegetable (AIV) greenhouses to be optimized under stringent production conditions to remain competitive. Currently, greenhouse management decisions are categorized into various levels, ranging from real-time control to environmental optimization and seasonal market planning. The primary objective of our research is to optimize greenhouse conditions, specifically temperature, humidity, and airflow, at the Agro Industrial Park (AIP). To accomplish this, Design-Expert software, which is a computer-based tool equipped with contemporary statistical models and tools, was employed to accurately estimate the required ranges of microclimate parameters that should be maintained to achieve optimal growth rate and yield. By utilizing this approach, this study aimed to provide valuable insights and recommendations for enhancing greenhouse productivity. Ultimately, this study contributes to the development and advancement of AIV greenhouses by offering practical solutions to optimize microclimate conditions, resulting in improved growth and increased yield.