To compare the antibacterial efficiency of MTAD, 2.5% NaOCl, and 2% CHX when used as root canal irrigants against Enterococcus faecalis, 40 extracted human maxillary central incisors were selected and decoronated. An inoculum of 10µl of 24-hour pure culture suspension of Enterococcus faecalis was inserted into the root canals of all teeth. After incubation, the teeth were randomly divided into 4 groups of 10 teeth in each group and instrumented using the step-back technique. During the process of instrumentation, respective root canal irrigants were used as per the groups: Group A - MTAD, Group B - 2.5% NaOCl, Group C - 2% CHX, and Group D - saline. Pre and post-treatment samples were obtained, and bacterial culture was carried out. Results and Conclusion: The results were statistically analyzed using SYSTAT software and the Kruskal Wallis test. There was a statistically significant difference between all the groups. Group A showed the maximum antibacterial efficiency, followed by Group B, Group C, and Group D.