Diversities in the internal anatomy are found in all teeth. Knowledge of these variations, particularly concerning the location and treatment of all canals, is essential for the success of endodontic therapy. Endodontic literature has suggested for ages that maxillary premolars are bicuspid teeth with one or two canals. The possibility of three roots and three canals in maxillary premolars is still rare. This case report presents a clinical case of a maxillary premolar with one palatal canal, one mesio-buccal canal, and one disto-buccal canal. This report serves as a reminder to clinicians that such anatomical variations should be taken into account during endodontic treatment of maxillary premolars.