This study aimed to evaluate the microtensile bond strength of two resin cements and a modified glass ionomer cement on both coronal and root dentin. Fifteen extracted molars were randomly divided into three groups of five teeth each. Rely X ARC (dual-cure resin cement), MULTILINK (self-cure resin cement), and FujiCEM (resin-modified glass ionomer cement) were used to measure microtensile bond strength. The results showed that Group I, Rely X ARC (10.6±0.9 Mpa), had the highest bond strength compared to Group II, MULTILINK (5.4±0.9 Mpa), and Group III, FUJICEM (3.8±0.7 Mpa). Although there were differences in microtensile bond strength among the tested materials, no significant difference was found between bond strengths to coronal and root substrates.