Neutrophil function plays a crucial role in the defense mechanisms against periodontal diseases. The malfunctioning of neutrophils leads to various destructive periodontal diseases. Therefore, it is important to diagnose these diseases accurately for effective management and prevention of tissue destruction. Neutrophil function tests have become an important tool in diagnosing the periodontal diseases. This review aims to provide an overview of the various neutrophil function tests, including chemotaxis, phagocytosis, degranulation, and oxidative burst. These tests are evaluated in vitro and in vivo using different techniques. The review also highlights the advantages and limitations of these tests, emphasizing the importance of proper interpretation and standardization of results. Finally, the review discusses the potential of using neutrophil function tests in monitoring the effectiveness of periodontal treatments and predicting the risk of future periodontal disease progression.