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In Vitro Anticancer and Antioxidant Activity Screening of Selected Marine Micro Algal Extracts

Rahul Pratap*, M. L. Lal Prasanth**, Anu V.***

*-*** Dr. Moopen's College of Pharmacy, Kerala, India.

Periodicity:January - April'2023
DOI : https://doi.org/10.26634/jchem.3.1.19293

Abstract

The present study was aimed to evaluate the anticancer and antioxidant activity of the five algal samples named as S–1 to S–5 isolated from the seawater collection of Muttom coastal region of Kanyakumari District. Mass production of micro algae was done by using sea water containing Walne's medium and each cultivated algae was isolated by centrifugation and extracted with different solvents viz., ethyl acetate, methanol and water. The extracts obtained were evaluated for in vitro anticancer and antioxidant activity. Anticancer activity was evaluated by MTT assay using the cell lines MCF7 and SKMEL. In this evaluation, extracts of all the algal samples showed anticancer activity against the selected cell lines, however, the methanol extract of S–2 sample showed significant activity comparing with other samples. Antioxidant activity was evaluated by DPPH, reducing power assay and superoxide radical scavenging assay methods. In this evaluation also, the methanol extract of S–2 sample showed a significant antioxidant activity in all the tested methods. These results are useful for further investigation in the future.

Keywords

Marine Microalgae, Extraction, Anticancer Activity, Antioxidant Activity.

How to Cite this Article?

Pratap, R., Prasanth, M. L. L., and Anu, V. (2023). In Vitro Anticancer and Antioxidant Activity Screening of Selected Marine Micro Algal Extracts. i-manager's Journal on Chemical Sciences, 3(1), 6-11. https://doi.org/10.26634/jchem.3.1.19293

References

[1]. Awah, F. M., & Verla, A. W. (2010). Antioxidant activity, nitric oxide scavenging activity and phenolic contents of Ocimum gratissimum leaf extract. Journal of Medicinal Plants Research, 4(23), 2479-2487.

[2]. Baba, S. A., & Malik, S. A. (2015). Determination of total phenolic and flavonoid content, antimicrobial and antioxidant activity of a root extract of Arisaema jacquemontii Blume. Journal of Taibah University for Science, 9(4), 449-454.

[3]. Batool, R., Khan, M. R., Sajid, M., Ali, S., & Zahra, Z. (2019). Estimation of phytochemical constituents and in vitro antioxidant potencies of Brachychiton populneus (Schott & Endl.) R. Br. BMC Chemistry, 13, 1-15.

[4]. Habu, J. B., & Ibeh, B. O. (2015). In vitro antioxidant capacity and free radical scavenging evaluation of active metabolite constituents of Newbouldia laevis ethanolic leaf extract. Biological Research, 48, 1-10.

[5]. Jha, R. K., & Zi-Rong, X. (2004). Biomedical compounds from marine organisms. Marine Drugs, 2(3), 123-146.

[6]. Kumar, K. A., Manickam, M. S., Sreejith, M., & Sebastin, V. (2021). In Vitro antioxidant and anthelmintic activity of the extracts of whole plant crotalaria biflora (L). Annals of the Romanian Society for Cell Biology, 25(4), 150-161.

[7]. Lauritano, C., Andersen, J. H., Hansen, E., Albrigtsen, M., Escalera, L., Esposito, F., ... & Ianora, A. (2016). Bioactivity screening of microalgae for antioxidant, antiinflammator y, anticancer, anti-diabetes, and antibacterial activities. Frontiers in Marine Science, 3, 68.

[8]. Maadane, A., Merghoub, N., Ainane, T., El Arroussi, H., Benhima, R., Amzazi, S., ... & Wahby, I. (2015). Antioxidant activity of some Moroccan marine microalgae: Pufa profiles, carotenoids and phenolic content. Journal of Biotechnology, 215, 13-19.

[9]. Mathan, S., Smith, A. A., Kumaran, J., & Prakash, S. (2011). Anticancer and antimicrobial activity of Aspergillus protuberus SP1 isolated from marine sediments of South Indian coast. Chinese Journal of Natural Medicines, 9(4), 286-292.

[10]. Mathan, S., Smith, A. A., Kumaran, J., & Prakash, S. (2013). Anti cancer activity of n-butanol extract of marine sediment fungi Aspergillus protuberus sp1 against dalton's ascitic lymphoma in mice. Journal of Pharmaceutical Sciences and Research, 5(3), 76-79.

[11]. Morales, J. L., Cantillo-Ciau, Z. O., Sánchez-Molina, I., & Mena-Rejón, G. J. (2006). Screening of antibacterial and antifungal activities of six marine macroalgae from coasts of Yucatan peninsula. Pharmaceutical Biology, 44(8), 632-635.

[12]. Rahul, P., Rakesh, K. J., & Prasanth, M. L. (2021). Evaluation of antimicrobial activity of the extracts of selected marine microalgae against human pathogens. World Journal of Pharmaceutical and Medical Research, 7(1), 156-159.

[13]. Ruskin, R. S., Kumari, B. V., & Citarasu, T. (2017). In vitro antioxidant activity of various leaf extracts of canthium coromandelicum (burm. f.) alston. Asian Journal of Pharmaceutical and Clinical Research, 10(5), 214-218.

[14]. Saranya, C., Hemalatha, A., Parthiban, C., & Anantharaman, P. (2014). Evaluation of antioxidant properties, total phenolic and carotenoid content of Chaetoceros calcitrans, Chlorella salina and Isochrysis galbana. International Journal of Current Microbiology and Applied Sciences, 3(8), 365-377.

[15]. Sebastin, V., Gopalakrishnan, G., Sreejith, M., & Anoob Kumar, K. I. (2019). Preliminary phytochemical evaluation and spectral characterization of active constituents in the dried extracts of the whole plant Argyreia imbricata (Rox) Sant & Patel. Asian Journal of Pharmaceutical and Clinical Research, 12(11), 56-60.

[16]. Sebastin, V., Gopalakrishnan, G., Sreejith, M., & Kumar, A. K. I. (2021). Determination of total phenolic content and antioxidant activity of the whole plant extracts of Argyreia imbricata (Roth) Sant. & Patel. International Journal of Research in Pharmaceutical Sciences, 12(1), 919-925.

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