Hydroxyapatite [Ca10(PO4)6(OH)2; abbreviated as HAp] is an inorganic compound whose chemical composition is similar to the composition of bone. It is a material used for biomedical applications such as a bone substitute material in orthopedics and dentistry due to its excellent biocompatibility, bioactivity and osteoconduction properties. The aim of the research is to investigate the possibility of extracting hydroxyapatite powder from the bovine’s bone. All the tests were carried out on the powder prepared by burning bovine’s femur and then using heat treatment for the bone ash. X-ray diffraction analysis, Fourier transform infra-red spectroscopy, particle size analyzer, and atomic absorption spectroscopy were used to test the resulted powder. The results showed that the extracted white powder was hydroxyapatite and did not contain any other organic components of the bone.