Swine flu is a highly contagious disease caused by one of several influenza, such as influenza type A, B and C. It is a fast spreading disease all over the country in recent years. This study determines the effect of Structured Teaching Programme (STP) on the knowledge of prevention of Swine Flu H₁N₁ among the rural people in Maharashtra. In this study, pre-test and post-test study design was adopted. Total 50 participants were selected by using convenience sampling technique. The pre-test was conducted by using structured questionnaire on knowledge of prevention of Swine Flu. Then a 45 minute Structured Teaching Programme (STP) on knowledge of prevention of Swine Flu was conducted, and after 7 days, post test was conducted by using same structured questionnaire on knowledge of prevention of Swine Flu. The collected data were analyzed by using descriptive and inferential statistics. The study findings showed that in pre-test 25 (50%) respondent had good knowledge of prevention of Swine Flu whereas 33(66%) respondent had good knowledge of prevention of Swine Flu in post-test. The study concluded that Structured Teaching Programme (STP) was effective to improve knowledge level of respondents in rural population. There is a need to plan and implement Structured Teaching Programme to any epidemic disease by nurses to improve knowledge among the people.