Urinary catheterization, a procedure in which a tube is placed into the urinary bladder through urethra for draining out urine is the major cause of catheter associated infections that causes 70% to 80% of catheter associated infections globally, along with 17.5% infections in European hospitals, 23.6% in US hospitals and 52% in the hospitals of Pakistan. Objectives of the study were, to asses nurse's knowledge regarding urethral catheter related infections. This study was channelized in public tertiary care hospitals of Peshawar, Pakistan. A descriptive cross-sectional design was used for this study, and conveniently ninety nurses were selected for the study. The entire period of study was from September 2017 to December 2017. A self-developed questionnaire was used to assess knowledge. Out of 90 participants, GNM were 65 (72.2%) & BSc(N) 25 (27.8%). 61.1% of the participants had low level of average knowledge and 38.9% of them had high level of average knowledge. Nurses' knowledge was low, therefore the administration & Human Resource Department of the hospitals provide periodic in-service education, up-to-date guidelines to nurses which are evidence-based for catheter use, insertion, maintenance and extraction.