More women in India die from cervical cancer than in any other country. Cervical cancer is a preventable disease which can be detected by regular screening tests and follow up. The aim of the study was to assess the knowledge, practice and perceived barriers of screening among non-professional working women on prevention of cervical cancer. A descriptive study was conducted among 60 non-professional working women. convenience sampling technique were used to select the samples . Structured questionnaire were used to assess knowledge, practice and perceived barriers for screening cervical cancer .The data was analyzed by using descriptive and inferential statistics.The findings of sociodemographic and clinical data variables shows that 22 (36.7%) of the women were in the age group of 51 to 55 years, more than half 32(53.3%) had high school education, majority of the women 38 (63.3%) had family Income below Rs 16000,majority of women 42 (70%) were the resident of urban area, most of them 44 (73.3%) were married, majority 44 (73.4%) were having >15yrs of duration of marriage. Most of the samples 47 (78.3%) had inadequate level of knowledge, 11(18.3%) had moderate level of knowledge and only 2 (3.3%) had adequate level of knowledge. and 23 (38.3%) samples had adequate level of practice, nearly half of the samples 29(48.3%) had moderate level of practice and only 8 (13.3%) had inadequate level of practice on prevention of cervical cancer. More than the half 33(55%) had responded absence of symptoms were the perceived cognitive barriers, more than half of the women 35 (58%) had responded fear of finding cancer were the perceived emotional barriers and majority 46 (77%), had financial problem were the perceived practical barriers for screening of cervical cancer raised by the non-professional working women. There is a weak positive correlation between knowledge and practice on prevention of cervical cancer among non-professional working women.There is no significant association between the level of knowledge and practice on prevention of cervical cancer with selected socio demographic and clinical data variablesThe study concludes that non-professional working women have lack of knowledge and practice. Perceived barriers (cognitive, emotional and practical) were the reason for lowering the screening rates of cervical cancer. This indicates that extensive education is needed to improve the knowledge and screening practice among lower levels of women for early identification and prevention of cervical cancer.