Human papilloma virus is the most common sexually transmitted infection in the United States with 14 million persons newly diagnosed each year. Approximately 79 million people in the US currently have HPV with the majority of the cases in persons under 25 years old. Despite proven vaccine effectiveness in the reduction of HPV associated complications, adherence to recommended vaccination guidelines remains low. The purpose of this study was to identify the variables identified in the literature that can increase vaccination guideline compliance rates. The purpose of the HPV vaccine protocol was to positively affect HPV vaccine administration and HPV vaccine series completion. The study design was retrospective and quasi-experimental. The sample included voluntary participants in a privately owned family practice clinic with a patient population encompassing the life span in a rural area. Participants included healthcare providers, physicians, physician assistants and family nurse practitioners in a primary care setting. Methodology of the study included a pre-survey, educational intervention and post-surveys at timed intervals. A HPV vaccine protocol was implemented in the clinical setting using variables which included addressing lack of provider knowledge of HPV, failure of providers to recommend the vaccination series, the use of automated electronic reminders in achieving vaccination series compliance, and the importance of consistent provider recommendation to patients. At study completion, HPV vaccine series completion had increased from a baseline rate of 2.3% to 8.9%. Based on these findings, predetermined outcomes of a 10% increase in HPV vaccine administration and a 25% increase in HPV vaccine series initiation were achieved. In the first three months of the translational study, HPV vaccine administration (n=16 to n=35) increased by 118.75%. A 50% increase in knowledge among providers was not met despite statistically significant (p=.000) results between pre-survey and the second post-survey knowledge scores. A 50% increase in protocol utilization was not achieved among all providers during the study. Protocol utilization was measured at 18% at study completion. Study findings included that utilization of the HPV vaccine protocol did positively impact the HPV vaccine administration. The key finding of the study was that consistent provider recommendation is critical in HPV vaccine acceptance. The most significant results were related to knowledge gained in the clinical setting regarding HPV and the HPV vaccine. There was also a large increase in the number of HPV vaccines administered compared to the previous year within the clinical setting.