i-manager Publications

Neem Root Mediated-Biosynthesis of Silver Nanoparticles: Antimicrobial Activities and Application as Detergent Additive

Adelere Isiaka Adedayo*, Lateef Agbaje**, Adabara Nasiru Usman***, Babayi Hausatu****, Aboyeji David Oyeyemi *****

*,***-***** Department of Microbiology, School of Life Sciences, Federal University of Technology, Minna, Nigeria.

** Department of Pure and Applied Biology, Ladoke Akintola University of Technology, Ogbomoso, Nigeria.

Periodicity:October - December'2019
DOI : https://doi.org/10.26634/jls.1.1.16905

Abstract

This study reports on the biosynthesis of Silver nanoparticles (AgNPs) using aqueous extract of neem root. The synthesis was carried out by mixing 1mM silver nitrate with the aqueous extract of neem root (10:1) under ambient conditions. Characterization was done by UV- vis spectroscopy, Fourier Transform Infrared (FTIR) spectroscopy, and Scanning Electron Microscopy (SEM). The antibacterial and algicidal activities of bio-synthesized AgNPs were investigated against some clinical bacterial isolates and a bloom forming cyanobacterial strain, respectively. Their potential application as an antimicrobial detergent additive was evaluated. The bio-synthesized AgNPs displayed maximum absorbance at wavelength 425 nm. The particles are predominantly spherical in shape with size ranging from 20 to 60 nm. Data obtained from FTIR indicate that the protein molecules in extract played a very active role in the reduction of silver ions to form AgNPs. The particles demonstrated considerable antibacterial activities against clinical isolates of Escherichia coli, Streptococcus sp, and Klebsiella pneumoniae as they induced inhibition zone of 12-23 mm. The algicidal activity displayed by the particles against the bloom forming cyanobacterial strain was appreciable. Similarly, the incorporation of AgNPs as additive in the locally made detergent led to the total inhibition of growth of Escherichia coli, Klebsiella pneumoniae, Candida sp, and Aspergillus flavus. Therefore, results obtained in this study suggest the promising applications of the particles as an antimicrobial agent in the water treatment and drug development. The particles exhibited potential application as an antimicrobial additive in detergent production.

Keywords

Silver Nanoparticles, Bio-synthesis, Antimicrobial Activities, Algicidal Activities, Detergent Additive.

How to Cite this Article?

Adedayo, A. I., Agbaje, L., Usman, A. N., Hausatu, B., and Oyeyemi, A. D. (2019). Neem Root Mediated-Biosynthesis of Silver Nanoparticles: Antimicrobial Activities and Application as Detergent Additive. i-manager's Journal on Life Sciences, 1(1), 8-15. https://doi.org/10.26634/jls.1.1.16905

References

[1]. Adelere, I. A., & Lateef, A. (2016). A novel approach to the green synthesis of metallic nanoparticles: The use of agro-wastes, enzymes and pigments. Nanotechnol Review, 5(6), 567-587.

[2]. Adelere, I. A., Lateef, A., Aboyeji, D. O., Abdulsalam, R., Adabara N. U., & Bala, J. D. (2017). Biosynthesis of silver nanoparticles using aqueous extract of Buchholzia coriacea (wonderful kola) seeds and their antimicrobial activities. Annals Food Science and Technology, 18(4), 671-679.

[3]. Ahmad, A., Senapati, S., Khan, M. I., Kumar, R., Ramani, R., Srinivas, V., & Sastry, M. (2003). Intracellular synthesis of gold nanoparticles by a novel alkalotolerant actinomycete, rhodococcus species. Nanotechnology, 14(7), 824-828.

[4]. Anusha, L., Devi, C. S., & Sibi, G. (2017). Inhibition effects of cobalt nano particles against fresh water algal blooms caused by Microcystis and Oscillatoria. American Journal of Applied Scientific Research, 3(4), 26-32.

[5]. Asmathunisha, N., & Kathiresan, K. (2013). A review on biosynthesis of nanoparticles by marine organisms. Colloids and Surfaces B: Biointerfaces, 103, 283-287.

[6]. Basavaraja, S., Balaji, S. D., Lagashetty, A., Rajasab, A. H., & Venkataraman, A. (2008). Extracellular biosynthesis of silver nanoparticles using the fungus Fusarium semitectum. Materials Research Bulletin, 43(5), 1164-1170.

[7]. Bhat, R., Deshpande, R., Ganachari, S.V., Huh, D.O.,& Venkataraman, A. (2011). Photo-irradiated biosynthesis of silver nanoparticles using edible mushroom Pleurotus florida and their antibacterial activity studies. Bioinorganic Chemistry and Applications, 1-7.

[8]. Chaturvedi V., & Verma, P. (2015). Fabrication of silver nanoparticles from leaf extract of Butea monosperma (flame of forest) and their inhibitory effect on bloomforming cyanobacteria. Biores Bioproc,2(1), 1-8.

[9]. Creighton, J. A., Blatchford, C. G., & Albrecht, M. G. (1979). Plasma resonance enhancement of Raman scattering by pyridine adsorbed on silver or gold sol particles of size comparable to the excitation wavelength. Journal of the Chemical Society, Faraday Transactions II, 75, 790-798.

[10]. Devadiga, A., Shetty, K. V., & Saidutta, M. B. (2015). Timber industry wasteteak (Tectona grandis Linn.) leaf extract mediated synthesis of antibacterial silver nanoparticles. International Nano Letter, 5(4), 205-214.

[11]. Dua, V. K., Pandey, A. C., Raghavendra, K., Gupta, A., Sharma, T., & Dash, A. P. (2009). Larvicidal activity of neem oil (Azadirachta indica) formulation against mosquitoes. Malaria Journal, 8(1), 1-6.

[12]. Feng, Q. L., Wu, J., Chen, G. Q., Cui, F. Z., Kim, T. N., & Kim, J. O. (2000). A mechanistic study of the antibacterial effect of silver ions on Escherichia coli and Staphylococcus aureus. Journal of Biomedical Materials Research, 52(4), 662-668.

[13]. Girish, K., & Shankara, B. S. (2008). Neem–a green treasure. Electronic Journal of Biology, 4(3), 102-111.

[14]. Inbakandan, D., Kumar, C., Bavanilatha, M., Ravindra, D. N., Kirubagaran, R., & Khan, S. A. (2016). Ultrasonic-assisted green synthesis of flower like silver nanocolloids using marine sponge extract and its effect on oral biofilm bacteria and oral cancer cell lines. Microbial Pathogenesis, 99, 135-141.

[15]. Keat, C. L., Aziz, A., Eid, A. M., & Elmarzugi, N. A. (2015). Biosynthesis of nanoparticles and silver nanoparticles. Bioresources and Bioprocess, 2(1), 3-11.

[16]. Kuppusamy, P., Yusoff, M. M., Maniam, G. P., & Govinda, N. (2016). Biosynthesis of metallic nanoparticles using plant derivatives and their new avenues in pharmacological applications – an updated report. Saudi Pharmaceutical Journal, 24, 473-484.

[17]. Lateef, A. Adelere, I. A., Gueguim-Kana, E. B., Asafa, T. B., & Beukes, L. S. (2015). Green synthesis of silver nanoparticles using keratinase obtained from a strain of Bacillus safensis LAU 13. International Nano Letter, 5, 29-35.

[18]. Lateef, A., Azeez, M. A., Asafa, T. B., Yekeen, T. A., Akinboro, A., Oladipo, I. C., Azeez, L., Ojo, S. A., GueguimKana, E. B., & Beukes, L. S. (2016). Cocoa pod husk extractmediated biosynthesis of silver nanoparticles: its antimicrobial, antioxidant and larvicidal activities. Journal of Nanostructure and Chemistry, 6,159-169.

[19]. Liauw, J., Hoang, S., Choi, M., Eroglu, C., Choi, M., Sun, G. H., Percy, M., Wildman-Tobriner, B., Bliss, T., Guzman, R. G.,& Barres, B. A. (2008). Thrombospondins 1 and 2 are necessary for synaptic plasticity and functional recovery after stroke. Journal of Cerebral Blood Flow & Metabolism, 28(10), 1722-1732.

[20]. Madhumitha, G., & Roopan, S. M. (2013). Devastated crops: multifunctional efficacy for the production of nanoparticles. Journal of Nanomaterials, 1-12.

[21]. Mandal, S., Phadtare, S., & Sastry, M. (2005). Interfacing biology with nanoparticles. Current Applied Physics, 5(2), 118-127.

[22]. Priyadarshini, S., Gopinath, V., Priyadharsshini, N. M., Ali, D. M., & Velusamy, P. (2013). Synthesis of anisotropic silver nanoparticles using novel strain, Bacillus flexus and its application. Colloids and Surfaces B: Biointerfaces, 102, 232-237.

[23]. Raffi, M., Hussain, F., Bhatti, T. M., Akhter, J. I., Hameed, A., & Hasan, M. M. (2008). Antibacterial characterization of silver nanoparticles against E. coli ATCC-15224. Journal of Materials Science and Technology, 24(2), 192-196.

[24]. Roychoudhury, P., Bose, R., & Pal, R. (2018). Algicidal activity and DNA binding affinity of silver nanoparticlebiofabricated by green alga, rhizoclonium riparium. Journal of Algal Biomass Utilization, 9(1), 67-77.

[25]. Salem, W. M., Haridy, M., Sayed, W. F., & Hassan, N. H. (2014). Antibacterial activity of silver nanoparticles synthesized from latex and leaf extract of Ficus sycomorus. Industrial Crops and Products, 62, 228-234.

[26]. Selvi, K. V., & Sivakumar, T. (2012). Isolation and characterization of silver nanoparticles from Fusarium oxysporium. International Journal of Current Microbiology and Applied Science, 1(1), 56-62.

[27]. Shankar, S., Jaiswal, L., Aparna, R. S. L., & Prasad, V. (2014). Synthesis, characterization, in vitro biocompatibility, and antimicrobial activity of gold, silver and gold silver alloy nanoparticles prepared from Lansium domesticum fruit peel extract. Material Letters, 137, 75-78.

[28]. Song, H. Y., Ko, K. K., Oh, L. H., & Lee, B. T. (2006). Fabrication of silver nanoparticles and their antimicrobial mechanisms. Euro Cells and Materials, 11(1), 58-62.

[29]. Thirumurugan, A., Tomy, N. A., Kumar, H. P., & Prakash, P. (2011). Biological synthesis of silver nanoparticles by Lantana camara leaf extracts. International Journal of Nanomaterials and Biostructures,1(2), 22–24.

[30]. Zaki, S., El-Kady, M. F., & Abd-El-Haleem, D. (2011). Biosynthesis and structural characterization of silver nanoparticles from bacterial isolates. Materials Research Bulletin, 46, 1571–1576.

If you have access to this article please login to view the article or kindly login to purchase the article

Single Article

	North Americas,UK, Middle East,Europe		India	Rest of world
	USD	EUR	INR	USD-ROW
Online	15	15

ADD TO CART

Options for accessing this content:

If you would like institutional access to this content, please recommend the title to your librarian.
If you already have i-manager's user account: Login above and proceed to purchase the article.
New Users: Please register, then proceed to purchase the article.