Neem Root Mediated-Biosynthesis of Silver Nanoparticles: Antimicrobial Activities and Application as Detergent Additive

Adelere Isiaka Adedayo*, Lateef Agbaje**, Adabara Nasiru Usman***, Babayi Hausatu****, Aboyeji David Oyeyemi *****
*,***-***** Department of Microbiology, School of Life Sciences, Federal University of Technology, Minna, Nigeria.
** Department of Pure and Applied Biology, Ladoke Akintola University of Technology, Ogbomoso, Nigeria.
Periodicity:October - December'2019


This study reports on the biosynthesis of Silver nanoparticles (AgNPs) using aqueous extract of neem root. The synthesis was carried out by mixing 1mM silver nitrate with the aqueous extract of neem root (10:1) under ambient conditions. Characterization was done by UV- vis spectroscopy, Fourier Transform Infrared (FTIR) spectroscopy, and Scanning Electron Microscopy (SEM). The antibacterial and algicidal activities of bio-synthesized AgNPs were investigated against some clinical bacterial isolates and a bloom forming cyanobacterial strain, respectively. Their potential application as an antimicrobial detergent additive was evaluated. The bio-synthesized AgNPs displayed maximum absorbance at wavelength 425 nm. The particles are predominantly spherical in shape with size ranging from 20 to 60 nm. Data obtained from FTIR indicate that the protein molecules in extract played a very active role in the reduction of silver ions to form AgNPs. The particles demonstrated considerable antibacterial activities against clinical isolates of Escherichia coli, Streptococcus sp, and Klebsiella pneumoniae as they induced inhibition zone of 12-23 mm. The algicidal activity displayed by the particles against the bloom forming cyanobacterial strain was appreciable. Similarly, the incorporation of AgNPs as additive in the locally made detergent led to the total inhibition of growth of Escherichia coli, Klebsiella pneumoniae, Candida sp, and Aspergillus flavus. Therefore, results obtained in this study suggest the promising applications of the particles as an antimicrobial agent in the water treatment and drug development. The particles exhibited potential application as an antimicrobial additive in detergent production.


Silver Nanoparticles, Bio-synthesis, Antimicrobial Activities, Algicidal Activities, Detergent Additive.

How to Cite this Article?

Adedayo, A. I., Agbaje, L., Usman, A. N., Hausatu, B., and Oyeyemi, A. D. (2019). Neem Root Mediated-Biosynthesis of Silver Nanoparticles: Antimicrobial Activities and Application as Detergent Additive. i-manager's Journal on Life Sciences, 1(1), 8-15.


[2]. Adelere, I. A., Lateef, A., Aboyeji, D. O., Abdulsalam, R., Adabara N. U., & Bala, J. D. (2017). Biosynthesis of silver nanoparticles using aqueous extract of Buchholzia coriacea (wonderful kola) seeds and their antimicrobial activities. Annals Food Science and Technology, 18(4), 671-679.
[3]. Ahmad, A., Senapati, S., Khan, M. I., Kumar, R., Ramani, R., Srinivas, V., & Sastry, M. (2003). Intracellular synthesis of gold nanoparticles by a novel alkalotolerant actinomycete, rhodococcus species. Nanotechnology, 14(7), 824-828.
[4]. Anusha, L., Devi, C. S., & Sibi, G. (2017). Inhibition effects of cobalt nano particles against fresh water algal blooms caused by Microcystis and Oscillatoria. American Journal of Applied Scientific Research, 3(4), 26-32.
[13]. Girish, K., & Shankara, B. S. (2008). Neem–a green treasure. Electronic Journal of Biology, 4(3), 102-111.
[23]. Raffi, M., Hussain, F., Bhatti, T. M., Akhter, J. I., Hameed, A., & Hasan, M. M. (2008). Antibacterial characterization of silver nanoparticles against E. coli ATCC-15224. Journal of Materials Science and Technology, 24(2), 192-196.
[24]. Roychoudhury, P., Bose, R., & Pal, R. (2018). Algicidal activity and DNA binding affinity of silver nanoparticlebiofabricated by green alga, rhizoclonium riparium. Journal of Algal Biomass Utilization, 9(1), 67-77.
[26]. Selvi, K. V., & Sivakumar, T. (2012). Isolation and characterization of silver nanoparticles from Fusarium oxysporium. International Journal of Current Microbiology and Applied Science, 1(1), 56-62.
If you have access to this article please login to view the article or kindly login to purchase the article

Purchase Instant Access

Single Article

North Americas,UK,
Middle East,Europe
India Rest of world
Pdf 35 35 200 20
Online 35 35 200 15
Pdf & Online 35 35 400 25

Options for accessing this content:
  • If you would like institutional access to this content, please recommend the title to your librarian.
    Library Recommendation Form
  • If you already have i-manager's user account: Login above and proceed to purchase the article.
  • New Users: Please register, then proceed to purchase the article.