Susceptibility of Multidrug Resistant Bacteria to Lyophilized Citrus Limon Juice Extract

Adabara N. U. *, Abubakar I. **, Adedeji A. S. ***, Kuta F. A.****, Bala J. D. *****, Oyedum U. M. ******
*-****** Department of Microbiology, Federal University of Technology, Minna, Nigeria.
Periodicity:October - December'2019


This research was carried out to evaluate the antibacterial activity of lyophilized Citrus limon on multi-drug resistant Escherichia coli, Pseudomonas aeruginosa, Klebsiella pneumoniae, Salmonella typhi, Macrococcus luteus and Clostridium difficile. Phytochemical constituent of the lyophilized citrus limon was determined using standard laboratory methods, while the agar well diffusion technique was used to evaluate the antibacterial potential of the lyophilized extract. Phytochemistry of the lyophilized Citrus limon extract revealed the presence of saponins, tannin, flavonoid, glycoside, cardiac, terpenes, anthraquinone, resins phenol, alkaloid, volatile oils and steroid. Lyophilized Citrus limon juice extract had a significant activity (p<0.05) with zone of inhibition as follows 26.00±1.4, 21.00±1.41, 24.50±0.70, 16.50±0.70, 29.50±2.12 and 20.50±0.00 mm at 40 mg/mL against Escherichia coli, Pseudomonas aeruginosa, Klebsiella pneumonia, Salmonella typhi, Macrococcus luteus and Clostridium difficile, respectively. The minimum inhibitory concentration ranges from 10-80 mg/mL and the minimum bacteriocidal concentration ranges from 80-160 mg/mL. This study provides insight on the antibacterial potential of Citrus limon juice extract against multidrug resistant bacterial pathogens. It is recommended that further studies should be conducted to explore the Citrus limon bioactive compound in the development of therapeutic drug.


Citrus Limon, Therapeutic, Rutaceae, Lyophilized, Bacteriocidal.

How to Cite this Article?

Adabara N. U., Abubakar, I., Adedeji, A. S., Kuta, F. A., Bala J. D., and Oyedum U. M. (2019). Susceptibility of Multidrug Resistant Bacteria to Lyophilized Citrus Limon Juice Extract. i-manager's Journal on Life Sciences, 1(1), 1-7.


[3]. Chessbrough, M. (2006). District Laboratory Practice in Tropical Countries Part II (2nd ed.). Cambridge University press, United Kingdom.
[5]. Clinical and Laboratory Standard Institute. (2017). Performance Standards for Antimicrobial Susceptibility Testing (27th ed.). Wayne, Pennsylvania, USA.
[6]. Collins, C.H., Lyne, P.M., Grange, J. M., & Falkinham III, J. O. (2006). Microbiological Methods (7th ed, pp.81-156). Butterworth & Co.Publishers.
[11]. Mahajan, B.K. (1997). Significance of Differences in Means: Methods in Biostatistics for Medical and Research Workers (6th ed.). New Delhi: JAYPEE Brothers Medical.
[14]. Pandey, A., Kaushik, A., & Tiwari, S. K. (2011). Evaluation of antimicrobial activity and phytochemical analysis of Citrus limon. Journal of Pharmaceutical and Biomedical Sciences, 13(17), 1-7.
[15]. Parekh, J., Jadeja, D., & Chanda, S. (2006). Efficacy of aqueous and methanol extracts of some medicinal plants for potential antibacterial activity. Turkish Journal of Biology, 29(4), 203-210.
[17]. Sofowora, A.E. (1993). Medicinal Plants & Traditional Medicine in Africa. Nigeria: John Wiley & Sons, Ltd.
If you have access to this article please login to view the article or kindly login to purchase the article

Purchase Instant Access

Single Article

North Americas,UK,
Middle East,Europe
India Rest of world
Pdf 35 35 200 20
Online 35 35 200 15
Pdf & Online 35 35 400 25

Options for accessing this content:
  • If you would like institutional access to this content, please recommend the title to your librarian.
    Library Recommendation Form
  • If you already have i-manager's user account: Login above and proceed to purchase the article.
  • New Users: Please register, then proceed to purchase the article.