Adolescence is an important stage of life when a child matures into an adult. The onset of menstruation is one of the major physiological changes that occur in this stage during the adolescence of life. So, good menstrual hygiene is important for the better health and dignity of the adolescent girls. The study was conducted in Balangir, Odisha to assess the knowledge and practice of menstrual hygiene among adolescent girls. The quantitative research approach with descriptive (non experimental) research design was adopted with a sample size of 40 adolescent girls aged 13- 15 years th th th studying in 8th , 9th and 10th standards, who were purposively selected. The data was collected by using a self-structured knowledge and practice questionnaire on menstrual hygiene. Findings revealed that mean knowledge score of adolescent girls on menstrual hygiene was 10.32±1.66. According to knowledge and practice, 32(80%) were fair and 8(20%) had good knowledge whereas, 60% were satisfactory and 40% had unsatisfactory level of practice regarding menstrual hygiene. The study concludes that there is a need to increase knowledge on menstrual hygiene and also aware them about the good practice menstrual hygiene to ensure a better reproductive life.