Inconel 601 is a widely used material in the nuclear and aerospace industries due to its superior chemical and thermal properties. However, there has been relatively less study on the plasma nitriding of Inconel 601 at moderate temperatures. Therefore, the current study reports on the plasma ion nitriding of Inconel 601 alloys in the lowtemperature regime, ranging approximately from 350°C to 450°C. The development of the nitrided layer and its microstructure was examined using X-ray Diffraction (XRD) and Scanning Electron Microscope (SEM). XRD analysis was conducted using a laboratory machine (Panalytical X'Pert Pro) with a Cu-Kα X-ray source (1.54 Å). To assess the effect of plasma nitriding on the alloy's surface properties, Micro-hardness Measurements, and a Wear Test (pin on disk) were performed. It was concluded that the nitriding process enhances the surface hardness. The XRD measurements indicated that this was due to the formation of Chromium Nitride (CrN) and the epsilon (ε) phase on the nitrided surface.