Titanium alloys are difficult to cut materials due to their physical and mechanical properties. Cutting fluids are employed in machining in order to improve machinability, tribological characteristics, tool life, reduce tool wear, helps in carrying away the heat and debris produced during machining. The use of cutting fluids in machining leads to higher machining cost, hazards to operator and environmental issues. Alternatives have been investigated to reduce the use of cutting fluids in machining. One of the alternatives is machining with Minimum Quantity Lubrication (MQL), where the cutting fluid flow rate is 10 to 500 ml/hr. Tool wear plays an important role on the components like surface finish, dimensional accuracy and cost of tools. In order to minimize the tool wear, it is required to select proper cutting fluid, optimize the process parameters such as cutting speed, feed rate and depth of cut while machining on titanium alloys. This paper deals the effect of process parameters and optimization of process parameters in turning of Ti-6Al-4V alloy under different cutting fluids such as Vegetable, Synthetic and Servo cut oils with Minimum Quantity Lubrication (MQL) and found that with synthetic oil, tool wear is low compared to vegetable and servo cut oils. It is observed that the high cutting speed at 580 rpm, moderate feed rate at 0.167 mm/rev and high depth of cut at 1.6 mm are found to be optimum cutting conditions. From ANOVA, It is observed that feed rate has major contribution in optimization of process parameters.