To perform the seismic assessment of reinforced concrete structures, the initial stage is to develop the seismic fragility function and use it to construct the fragility curve to determine the probability of failure of structures during earthquake. The Reinforced concrete structural wall with increase in number of stories is considered under simulated earthquake loading to establish the Peak Ground Acceleration (PGA)-based seismic fragility functions. Time history analysis method is used for developing the seismic fragility function and curves of low to mid-rise Reinforced Concrete (RC) structural walls. Time history method is also used to safeguard the safety of the RC structure from the damages due to earthquake. Time history analysis of the building or structure is done using ETABS software. A MATLAB code is used to plot the fragility curve for four damage states. As the earthquake causes huge damage to the reinforced concrete buildings or structures, it is essential to analyze and study the behavior of the building structure, whether it resists the damages caused to the structure due to shaking of the ground.