The use of dental hand-pieces endangers the dentist to ultra-vibrations. It is known that the vibration of dental handpieces is harmful to a long run in the frequency range above 1250 Hz. The daily endanger amplitude for dentist handarm vibrations shall be as per ISO 5349-1:2001. There is very less information available on the physical and other health issues about using dental hand-pieces. Till date, no study has been conducted in this area in the Asian continent. Handpieces are manufactured based on findings carried out on European dentists. The current study aims to study the exposure level of Indian dentists to vibration because of use of dental hand-pieces. A study to analyze the vibration transmissibility of hand-pieces used by the dentist during drilling operation is proposed. The current research work aims to analyze the vibration exposure and its transmissibility of Indian dentists during the drilling operation. The objective of this research is to study the change in the behaviour of vibration during idling to drilling in different conditions. The measuring instrument was biometric setup which includes; the accelerometer, the conditional amplifier, and the analysis software. The total number of hand-pieces analyzed in all directions were 20 during idling. The work was also studied by drilling on actual teeth and mandible in the skull. The weighted vibrations were also compared to standard ISO 5349:2001-1. Coolant injection had the least effect on the vibration transmissibility of dental hand-pieces. Under idling condition, the weighted vibrations transmissibility of used micro-motor hand-pieces was higher compared to new ones and also the weighted vibrations transmissibility of new and used air-turbine hand-pieces was nearly of the same amplitude. Drilling on the teeth did increase the vibration amplitude and transmissibility of micro-motor and air-turbine hand-pieces. But the effect of drilling is more on micro-motor hand-pieces than on air-turbine hand-pieces.