Road Safety Audit is a formal procedure for assessing accident potential and safety performance of new and existing roads. Road safety Audit is an efficient, cost effective and proactive approach to improve road safety and it has potential to save lives. The frequency of traffic collision in India is amongst the highest in the world. In 2015, one person dies every 4 minutes in road accidents in India. The objective of the study is to present some basic concepts of Road safety Audit for the major roads in Bengaluru in the state of Karnataka in India. The numbers of road accidents in Bengaluru are about 10% of the total accidents in Karnataka. Hence, there is a requirement of Road Safety Audit to reduce the accidents. The Road Safety Audit is aimed at reducing the injuries, fatalities, deaths, etc., due to road accidents. In order to develop road safety measures, it is required to collect accidents’ data, traffic volume data, and analyzing the data to identify the Black Spots and ranking the black spots based on the deficiencies of safety measures and identify most accident prone location by Equivalent Property Damage Method (EPDO). At black spots, the cause for the accident is identified and remedial measures are proposed to reduce the fatality and the number of accidents. After conducting the Road Safety Audit, it is found that the vehicles parked on road side reduces the effective width of road and affect the fast moving traffic, and the location of bus stop also affect the traffic. Along with this, the road geometry and the poor sign boards will also affect the safety measure.