This research explores the sustainability of bio-concrete in marine environments, focusing on its workability, compressive strength, and self-healing capabilities compared with conventional concrete using Bacillus subtilis bacteria. A total of 27 cubes were cast to study compressive strength and the self-healing mechanism of bio-concrete. Samples were cured in both normal water and marine water. After 28 days of curing, microcracks were induced in the cubes by applying one- third of the load, and the specimens were then exposed again to normal and marine water to evaluate healing. The findings demonstrate a compelling self-healing mechanism, with bio-concrete showing remarkable recovery in both curing conditions. Notably, the healing process was faster and more efficient in marine water, highlighting the adaptability of bio-concrete in harsh environments. Overall, the study emphasizes bio-concrete as a sustainable alternative for marine infrastructure, offering improved workability, enhanced compressive strength, and efficient self- healing, thereby contributing to the durability and resilience of coastal structures.