A Comparative Study of Using Diode Laser, Ultrasound and Alternating Current in Wound Healing

*, Eman Gh. Khalil**, Mays I. Khudhair***
* Professor, Department of Biomedical Engineering, College of Engineering, Al-Nahrain University, Iraq.
** Lecturer, Department of Biomedical Engineering, College of Engineering, Al-Nahrain University, Iraq.
*** Assistant Lecturer, Department of Biomedical Engineering, College of Engineering, Al-Nahrain University, Iraq.
Periodicity:February - April'2017
DOI : https://doi.org/10.26634/jfet.12.3.13430

Abstract

The laser, ultrasound, and electrical current energies are widely applied in the healing processes as well as in many other therapeutic applications in medicine. This study was established to evaluate the role of laser, ultrasound, and electric current on the wounds healing process and compare the results according to histological parameters of healing. Forty eight male mice of 20-25 gm in weight were randomly subdivided into four groups of twelfth animals each as follows: Laser group, ultrasound group, electrical current group, and control group. A one centimeter linear incision was made on the shaved backs of all mice. Group 1 had given continuous diode laser with a wavelength of 637 nm, 250 mW power. 2 Group 2 had treated with 1 MHz ultrasound frequency, 0.5 W/cm intensity. Group 3 had submitted to pulsed biphasic rectangular current of 30 Hz frequency, 1 mA current. Group 4 was kept as control. The treatments were applied at the same day, three sessions/week, for two weeks. The results were collected and compared histologically for certain days during the healing process. This study showed that, both electric current treatment and laser treatment have beneficial effects in the inflammatory and proliferation phases of wound healing, as compared with ultrasound treated group and control group.

Keywords

Diode Laser, Ultrasound, Electrical Current, Wound Healing

How to Cite this Article?

Dawood, M.S., Khalil, E.G., and Khudhair, M.I., (2017). A Comparative Study of Using Diode Laser, Ultrasound and Alternating Current in Wound Healing. i-manager’s Journal on Future Engineering and Technology, 12(3), 10-20. https://doi.org/10.26634/jfet.12.3.13430

References

[1]. M.E. Chaves, A.R. Araújo, A.C. Piancastelli, and M. Pinotti, (2014). “Effects of low-power light therapy on wound healing”. Anais Brasileiros de Dermatologia Journal, Vol. 89, No. 4, pp. 616–623.
[3]. R.A. Al-Magsosy, (2005). Design and Construction of a System to Study the Effect of Laser Radiation on the Absorbtion of Antibiotics in Blood. (M.Sc. Thesis, Al-Nahrain University, Iraq).
[4]. N. Herascu, B. Velciu, M. Calin, D. Savastru, and G. Yalianu, (2005). “Low level laser therapy LLLT efficacy in post-operation wounds”. Photomed Laser Surg., Vol. 23 No.1, pp. 70–73.
[5]. H. Tuby, L. Maltz, and U. Oron, (2006). “Modulations of VEGF and iNOS in the rat heart by low level laser therapy are associated with cardioprotection and enhanced angiogenesis”. Lasers Surg. Med., Vol. 38, No. 7, pp. 682- 688.
[6]. M.S. Dawood, and S.D. Salman, (2012). Low Level Diode Laser Accelerates Wound Healing. Springer-Verlag London.
[7]. M.A. Calin, T. Coman, and M.R. Calin, (2010). “The effect of low level laser therapy on surgical wound healing”. Romanian Reports in Physics, Vol. 62, No. 3, pp. 617–627.
[8]. M.S. Dawood, M.F. Al-Habeeb, and S.D. Salman, (2009). “A histological study of the effect of low level laser therapy on wound healing”. Al-Nahrain University College of Engineering Journal, Vol. 12, No.1, pp. 80–87.
[10]. D. Miller, N. Smith, M. Bailey, G. Czarnota, K. Hynynen, and I. Makin, (2012). “Overview of Therapeutic Ultrasound Applications and Safety Considerations”. J. Ultrasound Med., Vol. 31, No. 4, pp. 623–634.
[12]. D.C. Hocking, C.H. Raeman, and D. Dalecki, (2014). “Investigation of effects of ultrasound on dermal wound healing in diabetic mice”. The Journal of the Acoustical Society of America, Vol. 135, No. 4, pp. 2370-2370.
[15]. P. Scarborough, and L.C. Kloth, (2012). “EStimulation: An Effective Modality to Facilitate Wound Healing”. Today's Wound Clinic, Vol. 6, No. 4, pp. 28-32.
[16]. R.C. Araújo, P.M. Franciulli, R.O. Assis, R.R. Souza, and L. Mochizuki, (2007). “Effects of laser, ultrasound and electrical stimulation on the repair of achilles tendon injuries in rats: A comparative study”. Brazilian Journal of Morphological Sciences, Vol. 24, No. 3, pp. 187-191.
[17]. B.M. Bates, (2011). Wound Care: A Collaborative Practice Manual for Health Professionals, 4th Edition. Lippincott Williams & Wilkins
[18]. J.S. Kawalec, V.J. Hetherington, T.C. Pfennigwerth, D.S. Dockery, and M. Dolce, (2004). “Effect of a Diode Laser on Wound Healing by using Diabetic and Nondiabetic Mice”. The Journal of Foot & Ankle Surgery, Vol. 43, No. 4, pp. 214-220.
[19]. S. Kandela, and A. Kantrajian, (1988). “Laser light in enhancing wound healing rate”. Journal of Al-Nahrain University, Vol. 2, No. 4, pp. 80-88.
[20]. F.A. Watban, and X.Y. Zhang, (2004). “The comparison of effects between pulsed and CW lasers on wound healing”. Journal of Clinical Laser Medicine & Surgery, Vol. 22, No. 1, pp. 8-15.
[21]. H. Demir, S. Yaray, M. Kirnap, and K. Yaray, (2004a). “Comparison of the effects of laser and ultrasound treatments on experimental wound healing in rats”. Journal of Rehabilitation Research & Development, Vol. 41, No. 5, pp. 721–728.
[22]. A. Bell, and J. Cavorsi, (2008). “Noncontact Ultrasound Therapy for Adjunctive Treatment of Nonhealing Wounds: Retrospective Analysis”. Journal of the American Physical Therapy Association, Vol. 88, No. 12, pp. 1517–1524.
[23]. M. Brown, M.K. McDonnell, and D.N. Menton, (1988). “Electrical Stimulation effects on Cutaneous Wound in Rabbits”. Journal of the American Physical Therapy Association, Vol. 68, No. 6, pp. 955-960.
[24]. S.E. Gardner, R.A. Frantz, and F.L. Schmidt, (1999). “Effect of electrical stimulation on chronic wound healing: A meta-analysis”. Wound Repair Regeneration, Vol. 7, No. 6, pp. 495–503.
[25]. K.M. Bogie, S.I. Reger, S.P. Levine, and V. Sahgal, (2000). “Electrical stimulation for pressure sore prevention and wound healing”. Assistive Technology, Vol. 12, No. 1, pp. 50-66.
[26]. R. Ogrin, P. Darzins, and Z. Khalil, (2009). “The use of Sensory Nerve Stimulation and Compression Bandaging to improve Sensory Nerve Function and Healing of Chronic Venous Leg Ulcers”. Current Aging Science, Vol. 2, No. 1, pp. 72-80.
[27]. R.P. Abreu Freitas, and A.M. Barcelos, (2013). “Lowlevel laser therapy and micro current in burn wound healing in rats”. Fisioter. Pesqui., Vol. 20, No. 1, pp. 24- 30.
[28]. K. Chawla, A.K. Lamba, S. Tandon, F. Faraz, and V. Gaba, (2016). “Effect of low level laser therapy on wound healing after depigmentation procedure: A clinical study”. Journal of Indian Society of Periodontology, Vol. 20, No. 2, pp. 184-188.
[29]. R.V. Gonçalves, R.D. Novaes, M.C. Cupertino, B. Moraes, J.P. Leite, M.C. Peluzio, M.V. Pinto, and S.L. Matta, (2013). “Time-dependent effects of low-level laser therapy on the morphology and oxidative response in the skin wound healing in rats”. Lasers in Medical Science, Vol. 28, No. 2, pp. 383–390.
[30]. H. Demir, H. Balay, and M. Kirnap, (2004b). “A comparative study of the effects of electrical stimulation and laser treatment on experimental wound healing in rats”. Journal of Rehabilitation Research & Development, Vol. 41, No. 2, pp. 147–154.
If you have access to this article please login to view the article or kindly login to purchase the article

Purchase Instant Access

Single Article

North Americas,UK,
Middle East,Europe
India Rest of world
USD EUR INR USD-ROW
Online 15 15

Options for accessing this content:
  • If you would like institutional access to this content, please recommend the title to your librarian.
    Library Recommendation Form
  • If you already have i-manager's user account: Login above and proceed to purchase the article.
  • New Users: Please register, then proceed to purchase the article.