Time taken by the hospital authority to understand the condition of the patient is crucial in saving a patient's life. So need for tracking the patient's health conditions such as Heart rate, body temperature, Blood Pressure (BP), ECG signal, fatigue, air lung volume and vital-signs like patient physical appearance are sent to the hospital. All these parameters are monitored in the hospital, and also can be accessed by Doctors from anywhere. The system will allow emergency room doctors to get a real-time overview of patient's condition prior to their arrival at the hospital. This will help them to be prepared in advance to treat the patients once they arrive. This also helps them in proper intervention and intensive patient monitoring accordingly. In this paper, a real-time patient monitoring system in the ambulance while in transit is designed to transmit all the above patient vital parameters to the Hospital and the Doctor. The algorithms were also implemented to detect the abnormalities in these parameters. The system is implemented using LabVIEW real-time environment and Bio-sensors are interfaced through signal conditioning circuit to NI USB – 6281. This paper is a part of real time project funded by UGC India.