Yeast Recovery in Batch Ethanol Fermentation

Hynne Watsons Mutambandiro*, Itai Mutadza**, Asky T. Fungura***
*-*** Department of Chemical and Process Systems Engineering, Harare Institute of Technology, Zimbabwe.
Periodicity:October - December'2019
DOI : https://doi.org/10.26634/jchem.1.1.16427

Abstract

Yeast recovered after the fermentation of sugars can be reused again to ferment another batch. Based on the quantity of yeast used during fermentation, the amounts that can be recovered from these industries can be enormous. This experiment sought to prove that yeast derived after fermentation of sucrose still have good yeast count and viability of the quantities are just enough for pitching. The settled yeast from the beer well was collected and prepared in propagation vessels, where urea, zinc sulfate, magnesium sulfate, vitamins, ecozyme and food grade phosphoric acid were added. These were the nutrients needed for the growth and multiplication of yeast cells. The recovered yeast from the two fermenters Fv4 and Fv5 has an initial yeast count of 2x108 cell/mL and 2.9x108 cell/mL respectively. Initial viabilities for both samples were at 100%. Experiments are focused on the fermentation of the recovered yeast, which gave an average of 8% v/v ethanol. Therefore, harvesting yeast has a potential to be reused again in fermentation processes thereby reducing costs on procuring fresh yeast for each batch.

Keywords

Yeast, Fermentation, Propagation, Viability.

How to Cite this Article?

Mutambandiro, H. W., Mutadza, I., and Fungura, A. T. (2019). Yeast Recovery in Batch Ethanol Fermentation. i-manager's Journal on Chemical Sciences, 1(1), 1-7. https://doi.org/10.26634/jchem.1.1.16427

References

[1]. Amorim, H. V. D., Basso, L. C., & Lopes, M. L. (2004). Evolution of ethanol fermentation in Brazil. Distilled Spirits: Tradition and Innovation, 143-148.
[2]. Basílio, A. C. M., De Araújo, P. R. L., De Morais, J. O. F., da Silva Filho, E. A., De Morais, M. A., & Simões, D. A. (2008). Detection and identification of wild yeast contaminants of the industrial fuel ethanol fermentation process. Current Microbiology, 56(4), 322-326.
[3]. Basso, L. C., Basso, T. O., & Rocha, S. N. (2011). Ethanol production in Brazil: The industrial process and its impact on yeast fermentation. Biofuel Production-Recent Developments and Prospects, 1530, 85-100.
[4]. Basso, L. C., De Amorim, H. V., De Oliveira, A. J., & Lopes, M. L. (2008). Yeast selection for fuel ethanol production in Brazil. FEMS Yeast Research, 8(7), 1155-1163.
[5]. Bayrock, D. P., & Ingledew, W. M. (2001). Application of multistage continuous fermentation for production of fuel alcohol by very-high-gravity fermentation technology. Journal of Industrial Microbiology and Biotechnology, 27(2), 87-93.
[7]. Charoenchai, C., Fleet, G. H., & Henschke, P. A. (1998). Effects of temperature, pH, and sugar concentration on the growth rates and cell biomass of wine yeasts. American Journal of Enology and Viticulture, 49(3), 283-288.
[9]. Dien, B. S., Cotta, M. A., & Jeffries, T. W. (2003). Bacteria engineered for fuel ethanol production: Current status. Applied Microbiology and Biotechnology, 63(3), 258-266.
[10]. Fleet G. H., & Heard G. M. (1993). Yeasts-growth during fermentation: In Wine Microbiology and Biotechnology. Fleet GH. (ed). Harwood Academic: Switzerland, 42-43.
[11]. Kasavi, C., Finore, I., Lama, L., Nicolaus, B., Oliver, S. G., Oner, E. T., & Kirdar, B. (2012). Evaluation of industrial Saccharomyces cerevisiae strains for ethanol production from biomass. Biomass and Bioenergy, 45, 230-238.
[15]. Matta, V. M., & de Andrade Medronho, R. (2000). A new method for yeast recovery in batch ethanol fermentations: filter aid filtration followed by separation of yeast from filter aid using hydrocyclones. Bioseparation, 9(1), 43-53.
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