JIP_V3_N2_RP5 Prakriti Gautam Deepak Sharma Journal on Image Processing 2349-6827 3 2 35 40 Steganography, Various Techniques, MLSB, DCT, JPEG, BMP, PNG, TIFF, GIF, PSNR, MSE Steganography is the process of hiding information in a carrier in order to provide the secrecy of text, music, audio and images. It can be defined as the study of imperceptible communication that deals with the ways of concealing the existence of communicating messages. For hiding secret information in various file formats, there exists a large variety of Steganographic techniques where some are more complex than others and all of them have respective strong and weak points. The aim of the paper is to provide the user, a comparative analysis of the first and second level steganography using the MLSB embedding technique in various types of file formats as the cover image like JPEG, BMP, PNG, etc. With the help of results obtained, the best format for both the levels of hiding can be concluded. The secret image used is also taken of all different formats so that a conclusion can be drawn that which type of format is suitable for the cover as well as secret image. April - June 2016 Copyright © 2016 i-manager publications. All rights reserved. i-manager Publications http://www.imanagerpublications.com/Article.aspx?ArticleId=6028